Device

Part:BBa_M36284:Design

Designed by: Sunil Bodapati, Julia Joung   Group: Stanford BIOE44 - S11   (2011-11-29)


Human Alcohol Dehydrogenase 1B actuator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 463
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 463
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 463
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 463
    Illegal NgoMIV site found at 981
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We maximized expression by using a highly expressing bicistronic RBS. We needed a way to purify the enzyme post production. The histidine tag allows us to perform nickel affinity chromatography to isolate purified protein.


Source

Bicistonic RBS - BioFab: apFAB563 ADH1B - parts.igem: BBa_M36666 Histidine Tag - 6 histidines Stop Codon - generic TAG Terminator - BIOFAB: apFAB391

References